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Figure 2 | BMC Research Notes

Figure 2

From: Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella species

Figure 2

Melt curves of one HRM assay with titrations of target and non target DNA. The results of a titration range of 1 ng-100 fg genomic DNA from B. melitensis 16 M (red) and B. abortus 544 (blue) using the B. melitensis HRM assay and the HRM melt curves directly. Curves move from right to left with decreasing DNA concentration. As shown, the curves generated by very low concentrations of B. abortus DNA are very close to the curves generated by high concentrations of B. melitensis DNA and could be misidentified through this association.

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BMC Research Notes

ISSN: 1756-0500

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