Table 3 Characterization of the 72 polymorphic SSR markers developed for U. humidicola
SSR locus | GenBank accession number | Repeat motif | Ta (°C) a | Primer sequences (5′-3′) | Urochloa species accessions* | U. humidicola accessions** | ||||
|---|---|---|---|---|---|---|---|---|---|---|
Size range (bp) | A b | PIC c | A b | PIC c | DP d | |||||
BhUNICAMP068 | KM068303 | (CACACC)4(CA)17 | 58.5 | F_CCACAAACGTGAACACATACA R_AGGGACGGAAACACCCTTAG | 226-261 | 10 | 0.87 | 10 | 0.87 | 0.95 |
BhUNICAMP069 | KM068304 | (TC)25 | 64.5 | F_GAGGAACTCCTTTGGGTAGA R_TTCAGAGAGAGGATGGTATAGAG | 285-300 | 2 | 0.36 | 2 | 0.36 | 0.58 |
BhUNICAMP070 | KM068305 | (GT)9 | 65 | F_CCCCGGTCTCGACCTATC R_GAGGCTGCCCCCTTACTC | 174-214 | 12 | 0.84 | 6 | 0.78 | 0.54 |
BhUNICAMP071 | KM068306 | (AC)11 | 65 | F_CGCAACGAAGCTCCAATAG R_CGATCGCAAGCGTGTATCTA | 160-228 | 11 | 0.86 | 11 | 0.86 | 0.94 |
BhUNICAMP072 | KM068307 | (GT)7 | 56.5 | F_CCCCATGTAAACAACCGTAGA R_CCATGGTTGACCGCTAGAA | 174-186 | 3 | 0.56 | 3 | 0.56 | 0.85 |
BhUNICAMP073 | KM068308 | (TG)10 | 60 | F_TGAACATGTGAATGCCCACT R_ATTGCAGGATGCGGACTCTA | 240-304 | 10 | 0.85 | 10 | 0.85 | 0.94 |
BhUNICAMP074 | KM068309 | (CT)6 | 58.5 | F_ACGAACGATCCGACCAACTA R_TGCTTACGAGACGGCATAGA | 231-255 | 7 | 0.81 | 7 | 0.81 | 0.92 |
BhUNICAMP075 | KM068310 | (TC)22 | 50 | F_TGAATGCTTTTGTCCTGGTATC R_ACGTGCAGCAGCAACAGTA | 148-236 | 28 | 0.95 | 24 | 0.95 | 0.98 |
BhUNICAMP076 | KM068311 | (AC)18 | 51.5 | F_CCGATGGTCAAAGGTCAGTT R_GGTGGGCATATACCATGTTT | 206-234 | 10 | 0.84 | 10 | 0.84 | 0.66 |
BhUNICAMP077 | KM068312 | (AC)7 | 65 | F_CGGGAAGTCCTACTCCGTAA R_GGAGCTCAAGGTAGGGATTG | 212-230 | 8 | 0.83 | 8 | 0.83 | 0.93 |
BhUNICAMP078 | KM068313 | (GT)7 | 58.5 | F_ACCAGTGCACGTCTGAAAGA R_CGATCACTGCTGCGTCATA | 216-218 | 2 | 0.35 | 2 | 0.35 | 0.52 |
BhUNICAMP079 | KM068314 | (AG)12G(GA)17 | 62.5 | F_GGATTGAAAGTTGGAGCACA R_GCATGCTGTGAAGGAGGTTA | 180-222 | 17 | 0.92 | 17 | 0.92 | 0.96 |
BhUNICAMP080 | KM068315 | (GA)26 | 50 | F_CAAGCCTCTTCATGCAAGTAAC R_TGTCATACCCCCATGATTAAGA | 176-230 | 22 | 0.93 | 21 | 0.93 | 0.93 |
BhUNICAMP081 | KM068316 | (AGC)5ACAAT(CA)11 | 55 | F_CTGGCATGGGTCCCTTTAC R_TCTTCTTCCTCCAGCCACAT | 160-179 | 5 | 0.75 | 5 | 0.75 | 0.95 |
BhUNICAMP082 | KM068317 | (CA)23 | 60 | F_TTGCCGGGAACAGTTATACA R_GAAGCTCTATCAAACAGCCCT | 157-192 | 9 | 0.82 | 9 | 0.82 | 0.92 |
BhUNICAMP083 | KM068318 | (AG)22 | 56.5 | F_AAACATGCACCGTCATAACT R_GGGCTTGATTCATTTGTTA | 152-190 | 6 | 0.68 | 4 | 0.68 | 0.77 |
BhUNICAMP084 | KM068319 | (TG)15 | 65 | F_GGCGAAGACCATACCCTGTA R_TGCTGGTGGAAGAAGATGAA | 159-182 | 9 | 0.80 | 9 | 0.80 | 0.96 |
BhUNICAMP085 | KM068320 | (GT)9 | 60 | F_CGATTTATCGACGACCGAGT R_CCTTACTCGCAGGTCTGTCC | 158-171 | 5 | 0.76 | 5 | 0.76 | 0.64 |
BhUNICAMP086 | KM068321 | (TC)19 | 65 | F_AGTTGAATGGGCTGAACCAT R_TGCACTTCCAGGATCAGACA | 238-326 | 10 | 0.82 | 10 | 0.82 | 0.93 |
BhUNICAMP087 | KM068322 | (GT)10 | 50 | F_GGCCATTTCTAGCCAAACAA R_CCTTACTCGCAGGTCTGTCC | 240 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP088 | KM068323 | (TG)12 | 65 | F_AGAGGTTCCATGGACATTGC R_CTCATCAACAGACGCCTGAA | 178 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP089 | KM068324 | (AC)7 | 65 | F_CCGGATAGAAGGTCTGAACG R_AGTCGTCGAAGCGAGCTCTA | 175 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP090 | KM068325 | (CA)10 | 65 | F_CAGAGTAAGCTTCCGGGACA R_CGATTTATCGACGACCGAGT | 200-300 | 12 | 0.85 | 11 | 0.85 | 0.91 |
BhUNICAMP091 | KM068326 | (AC)8 | 65 | F_CTTGTGCCACTTCCACCTTT R_TCGTGTGGACACTTCCTCTG | 120-150 | 9 | 0.83 | 9 | 0.83 | 0.95 |
BhUNICAMP092 | KM068327 | (TG)6 | 65 | F_ATGCCTTGCTCCCACTAACA R_TAAATGCTCCAGCGACCTTC | 135-168 | 11 | 0.85 | 11 | 0.85 | 0.91 |
BhUNICAMP093 | KM068328 | (AAG)4 | 65 | F_GGAGCGCTAATTTCGTTCAG R_CCTCCGTTCTCGCTAATGAC | 230 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP094 | KM068329 | (TG)7 | 65 | F_TTGGAGCTTTCCCTAGCTCA R_GAACAAGAAGGGAGGAAGCA | 272-290 | 4 | 0.31 | 4 | 0.31 | 0.39 |
BhUNICAMP095 | KM068330 | (TC)16(TG)14 | 65 | F_GGGTTGGCCTACACACTGAT R_CGCACGACATTGATACCTTG | 268-320 | 6 | 0.75 | 6 | 0.75 | 0.92 |
BhUNICAMP096 | KM068331 | (TC)8TT(TC)40 | 65 | F_TGTTCTGCTCACTGGTTTGG R_TCAGCTCTCTACGGCTGGAT | 157-255 | 11 | 0.87 | 11 | 0.87 | 0.95 |
BhUNICAMP097 | KM068332 | (GT)6 | 65 | F_GCGAGCTACCGAGGTATTTG R_ACGTCAATGTCGAGCTTCCT | 129-148 | 5 | 0.69 | 5 | 0.69 | 0.80 |
BhUNICAMP098 | KM068333 | (GT)10(G)18 | 65 | F_GGACTGGTCGTCTTTCCATC R_GCTTTCTGCAAGCGGTAGAT | 250-312 | 9 | 0.85 | 9 | 0.85 | 0.95 |
BhUNICAMP099 | KM068334 | (CA)10TG(GA)10 | 65 | F_TTTGTGGCACCTGCAGAATA R_CGCTTCGTGCTGACAGATTA | 124-174 | 16 | 0.91 | 16 | 0.91 | 0.99 |
BhUNICAMP100 | KM068335 | (TG)12 | 65 | F_GCGCCATGGTTTCATCTATT R_GGTGGTTCCTCGTGTGAGAT | 178-219 | 7 | 0.79 | 7 | 0.79 | 0.98 |
BhUNICAMP101 | KM068336 | (TG)28 | 65 | F_GGTAAAGAAGGGCCGGACT R_GCATGGCATGTTCCTACTGA | 128-184 | 14 | 0.89 | 12 | 0.89 | 0.97 |
BhUNICAMP102 | KM068337 | (GCGA)4 | 65 | F_TGGTGGGCTCCACTATCTCT R_TCCGCCATCTCTCCTCTCT | 224-260 | 12 | 0.89 | 12 | 0.89 | 0.94 |
BhUNICAMP103 | KM068338 | (CT)22 | 65 | F_AGCTCTCCCGCCTCTCTCT R_CATCCACACCGTCTCTCTCA | 100-156 | 14 | 0.91 | 14 | 0.91 | 0.96 |
BhUNICAMP104 | KM068339 | (TG)26 | 60 | F_ACGACGACCTAATGGGTGAA R_ACCCAGCAACAAATCTCGTC | 190-274 | 15 | 0.87 | 13 | 0.87 | 0.96 |
BhUNICAMP105 | KM068340 | (AC)10ATACACACACAC(AG)53 | 50 | F_CTCCATCACGTGCTTGCTAA R_GTGTGATCGGCTGGAGATTT | 100-176 | 30 | 0.93 | 29 | 0.93 | 0.98 |
BhUNICAMP106 | KM068341 | (TTTGT)3 | 50 | F_GCTGTTCGGAGAGGAATCTG R_ATGAGAGGAGGGAAGGAAGG | 135-155 | 8 | 0.79 | 7 | 0.79 | 0.91 |
BhUNICAMP107 | KM068342 | (GA)18 | 50 | F_GGGTCAGTGTCGTCTCAGTTT R_CAGATTCCTCTCCGAACAGC | 118-190 | 26 | 0.94 | 26 | 0.94 | 0.98 |
BhUNICAMP108 | KM068343 | (CT)16 | 65 | F_TTGCCATTACTGGATCTGGA R_GCGCCACCCATAACTTAAA | 112-160 | 14 | 0.85 | 13 | 0.85 | 0.94 |
BhUNICAMP109 | KM068344 | (GT)9 | 60 | F_AGCGAGTCAAGCACAAGGAT R_GGGTCCAATCTCCCTCTCTC | 186-226 | 9 | 0.82 | 9 | 0.82 | 0.93 |
BhUNICAMP110 | KM068345 | (TG)8 | 65 | F_TCTGCATCCACTAGGCTCAG R_TCCTCCACCTTCTTTCCGTA | 148-164 | 4 | 0.39 | 4 | 0.39 | 0.46 |
BhUNICAMP111 | KM068346 | (TG)27 | 65 | F_AACTCCGACTATCTTCCAGTTGA R_AATGCATGGGTAGGATCTGC | 250-330 | 15 | 0.89 | 15 | 0.89 | 0.96 |
BhUNICAMP112 | KM068347 | (AC)26 | 65 | F_GACCAAACCCTCCGAAGTTA R_GGTTGCAACTACACGACCAG | 246-300 | 10 | 0.81 | 10 | 0.81 | 0.94 |
BhUNICAMP113 | KM068348 | (CGTG)3 | 63 | F_AACTTCGAGAGGTTCGTCCA R_ACCGGCAATCTATCCGTGT | 144-179 | 3 | 0.45 | 3 | 0.45 | 0.51 |
BhUNICAMP114 | KM068349 | (CT)21 | 63 | F_TATACAAGGCGCATCCACAA R_GCTCTTTCCTCACGCTGTTC | 200-266 | 15 | 0.89 | 15 | 0.89 | 0.96 |
BhUNICAMP115 | KM068350 | (AC)27(AT)7 | 60 | F_CTTCCTGCCAATAAGCGAAG R_CGAGCTTCCAGATTCTTTGG | 240 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP116 | KM068351 | (TG)8 | 65 | F_CTCCGCACCGCTTAAATTAG R_GTTGGAAATGGTGCTTCCAC | 288-306 | 3 | 0.52 | 3 | 0.52 | 0.62 |
BhUNICAMP117 | KM068352 | (TGA)7 | 65 | F_CCAACTGAACGGCCATACTT R_CCCACAAAGGAACCCTGAT | 290-300 | 4 | 0.61 | 4 | 0.61 | 0.77 |
BhUNICAMP118 | KM068353 | (AG)9 | 50 | F_CTGCATAACTTTCAGCCATCTC R_TTGGCACAACTGGAACGTAG | 149 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP119 | KM068354 | (AAG)7 | 65 | F_AAGGGCGTGATGTTCTGAAG R_AGGCCAAACGAATTTCTCAA | 189-204 | 4 | 0.66 | 4 | 0.66 | 0.82 |
BhUNICAMP120 | KM068355 | (AT)8ACACACACACG(CA)9 | 65 | F_TCCAGCAGTGTGTTCCTCAG R_ACCAGGAGTGCATAGCCAAG | 190-200 | 6 | 0.71 | 6 | 0.71 | 0.75 |
BhUNICAMP121 | KM068356 | (TC)12 | 65 | F_CGCTACTGCTGCACACAAAT R_CTGAGTGCGCCGTATGTTTA | 170-195 | 6 | 0.71 | 6 | 0.71 | 0.92 |
BhUNICAMP122 | KM068357 | (GT)15 | 65 | F_AGGAAGGCTCGCACTCACTA R_CCAAAGGCGGTGGTTAGATA | 200-315 | 14 | 0.90 | 14 | 0.90 | 0.95 |
BhUNICAMP123 | KM068358 | (TTA)4 | 65 | F_CCAAACTCTAGCTTTCACAGCA R_TTGGATCCACGTCAAACAAG | 280 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP124 | KM068359 | (AG)23 | 65 | F_TTGGAGTTGCTGGGCTATTT R_GAACCAAGCATAAGGCAACA | 218-320 | 12 | 0.85 | 10 | 0.85 | 0.95 |
BhUNICAMP125 | KM068360 | (GT)8GAATGTGTGT(GA)7 | 65 | F_TGTTATCAGTGCAGGTGTTGG R_GAGGCTGACGAAAGCTCAAC | 258-280 | 7 | 0.81 | 7 | 0.81 | 0.93 |
BhUNICAMP126 | KM068361 | (AC)10 | 65 | F_GGGAACCCAGGGTATCGTAT R_CTCTCCCAGCGTCTTTCCTT | 210 | 1 | 0.00 | 1 | 0.00 | 0.00 |
BhUNICAMP127 | KM068362 | (GT)6 | 65 | F_CCACCATTGCTTCCAGAGTAA R_ATTCGCCTCTCCTAGCACAA | 272-320 | 7 | 0.69 | 7 | 0.69 | 0.91 |
BhUNICAMP128 | KM068363 | (GA)37 | 65 | F_TGCCTGGAGACTGAGAAAGG R_CCTGCAGCAGACCTTCACAT | 150-240 | 17 | 0.91 | 17 | 0.91 | 0.98 |
BhUNICAMP129 | KM068364 | (AC)7ATGAA(CATG)3(CA)22 | 63 | F_TGTGTTTAGACCGCCAACAA R_TTATCGGCTCCCATTCACTC | 207-310 | 11 | 0.84 | 10 | 0.84 | 0.95 |
BhUNICAMP130 | KM068365 | (AC)7 | 63 | F_ACGCAGGAGAACTGCGTATC R_ATGGGATCCAACCGAACATA | 236-300 | 12 | 0.79 | 11 | 0.79 | 0.87 |
BhUNICAMP131 | KM068366 | (AC)7(A)16 | 60 | F_CATCAGATGCCTCAAACAGC R_GCAGGTGTGCAGCAAATAGA | 184-238 | 14 | 0.87 | 14 | 0.87 | 0.93 |
BhUNICAMP132 | KM068367 | (TG)7(T)29 | 50 | F_TCACTAGTGCGTCTGCTGCT R_GCACTCCATTGCAGACCTAAG | 184-196 | 4 | 0.53 | 3 | 0.53 | 0.63 |
BhUNICAMP133 | KM068368 | (TG)10 | 50 | F_CATGACTTATGTCCTTGGTGGA R_TCGACAGTGGAGCCACAA | 114-162 | 19 | 0.89 | 16 | 0.89 | 0.97 |
BhUNICAMP134 | KM068369 | (CCGG)3 | 60 | F_CAAACGGAGGAAGAGAGACG R_GGTGTCAATGCAGCCAAGTA | 114-135 | 9 | 0.75 | 5 | 0.75 | 0.83 |
BhUNICAMP135 | KM068370 | (AG)27 | 65 | F_CATGAGCCATCTCGTTGTTG R_TGCATTGACTTGACGTCTCC | 176-260 | 14 | 0.90 | 9 | 0.90 | 0.91 |
BhUNICAMP136 | KM068371 | (AC)9(ACAA)3 | 50 | F_TCCTGGTAAAGTTCCTCGTCA R_ACAACAATGCACGTCGAGAA | 225-290 | 7 | 0.75 | 6 | 0.75 | 0.93 |
BhUNICAMP137 | KM068372 | (GA)23 | 65 | F_TAGGTTTGGGTGGCACTAGG R_CTCCATGCTGCGTTGCTAT | 258-320 | 11 | 0.85 | 9 | 0.85 | 0.91 |
BhUNICAMP138 | KM068373 | (T)12 | 60 | F_TGCTCATGTGGGTCACATTT R_TGTGTGCCTGTGTGATGCTA | 270-288 | 5 | 0.70 | 5 | 0.70 | 0.95 |
BhUNICAMP139 | KM068374 | (AAAAG)3 | 65 | F_TCCTTTCTTTGAGCCGAGAG R_GCTGATGCTGACATCAAGGA | 248-294 | 6 | 0.67 | 5 | 0.67 | 0.97 |
Total average | 10.26 | 0.77 | 9.60 | 0.77 | 0.87 | |||||
Lb-1 average | 11.05 | 0.79 | 10.48 | 0.79 | 0.87 | |||||
Lb-2 average | 9.18 | 0.75 | 8.40 | 0.75 | 0.86 | |||||