Table 2 Primer sequence, location and expected amplicon sizes for dog COX-1 ( PTGS1 ), COX-2 ( PTGS2 ) and HPRT1 genes
From: Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells
Gene | Accession | Primer name | Primer sequence | Amplicon (bp) | Location |
|---|---|---|---|---|---|
PTGS1 | NM_001003023 | Cox1_E10-11Â F | TGTCCTTCCAGGAACTCACA | 161 | Exon10 |
Cox1_E10-11R | GAAGGGAGCCCCAATTTCTA | Exon11 | |||
PTGS1 | NM_001003023 | Cox1_E8-9Â F | ATCCTCATTGGGGAGACCAT | 193 | Exon8-9 |
Cox1_E8-9R | AACCCACCCAGAAGGAGTCT | Exon9 | |||
PTGS1 | NM_001003023 | Cox1_E4-5Â F | CTTTCCTCCACTTCCTGCTG | 168 | Exon4 |
Cox1_E4-5R | AGAAGGACTCCCAGCTGATG | Exon5 | |||
PTGS2 | NM_001003354 | Cox2_E6-7Â F | GTTCATTCCTGATCCCCAAG | 186 | Exon6 |
Cox2_E6-7R | TTGAAAAGGCGCAGTTTATG | Exon7 | |||
HPRT1 | NM_001003357 | Hprt1_F | TGACACTGGGAAAACAATGCAGACT | 110 | Exon6 |
Hprt1_R | AGCCAACACTTCGAGGGGTCCT | Exon7 |