Fig. 3
From: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology
Western blot analysis of GFP (a) and OmpA (b) proteins using beads-immobilized DNA template. Proteins were detected using peroxidase-conjugated anti-polyHistidine monoclonal antibody. The calculated protein sizes for GFP and OmpA are 30 and 27 kDa, respectively. DNA templates used for cell-free expression were as follows: a Lane 1, gfp-SA beads template; lane 2, gfp-CA beads template; lane 3, soluble DNA coding for GFP (as positive control); lane 4, no DNA template (as negative control). b Lane 1, no DNA template (as negative control); lane 2, ompA-CA beads template; lane 3, ompA-SA beads template; lane 4, soluble ompA protein coding DNA (as positive control)