Fig. 1

IGF-IR is expressed in lung cancer cells and its activity is inhibited by treatment with BMS-754807. Western blots of pIGF-IR/IR, IGF-IR, pAKT, AKT, pERK and ERK in A549 (a, c) and NCI-H358 (b, d) cells 4 (a, b) or 24 (c, d) hours after administration of 0.25 or 0.5 μM of BMS-754807. The bar graphs (e–h) represent the quantification of three independent western blots with the bars representing the means and the error bars representing SEM. The protein levels were normalized to the DMSO control group for each protein; the no treatment group was not quantified. β-actin was used as a loading control in the western blots and *p < 0.05 as determined by a Dunnett’s test. The bands from the 4 h No Treatment groups were quantified and are presented as the mean ± SEM for each protein relative to β-actin to illustrate the differences in protein levels in A549 and NCI-H358 cells (i)