Fig. 1
From: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
Not all PCR mixes result in optimal specificity of cDNA amplification reactions. The performance of three commercial SYBR green PCR mixes was compared by amplifying cDNA with 70 primer-based assays targeting different mRNA transcripts. A single melting curve peak indicates specificity of the amplification. The figure shows examples of melting curves and corresponding gel blots for several primer sets from Table 3, in situations where all three mixes gave optimal results and where one or more mixes resulted in a suboptimal amplification reaction