Table 4 Growth conditions of MG1655 and C58

E. coli MG1655

Standard

Grown at 37 °C in LB media

Reached mid-log phase O.D. 600 nm 0.6 ~ 0.8

Cold shock

Grown at 15 °C for 4 h then grown at 37 °C in LB media

Reached half of O.D. 600 nm of Standard

Heat shock

Grown at 50 °C for 4 h then grown at 37 °C in LB media

Same as above

Low pH

Grown at 37 °C for 1 h in LB media with pH4.5 then grown at 37 °C in LB media

Same as above

UV treat

Exposed to UV light for 15 min then grown at 37 °C in LB media

Same as above

Low carbon

Grown at 37 °C in minimal C source MOPS media [49] (C- MOPS)

Same as above

Low nitrogen

Grown at 37 °C in minimal N source MOPS media (N- MOPS)

Same as above

Low C & N

Grown at 37 °C in minimal C and N source MOPS media (C-N- MOPS)

Reached quarter of O.D. 600 nm of Standard

Oxidative

Growth at 37 °C in 49 mL MOPS media with 400 μL 7 % Hydrogen peroxide

Reached half of O.D. 600 nm of Standard

Osmotic

Growth at 37 °C in 45 mL MOPS media with 6 mL 4 M Sodium Chloride

Same as above

A. tumefaciens C58

Standard

Grown at 28 °C in YEM media

Reached mid-log phase O.D. 600 nm 0.6 ~ 0.8

Cold Shock

Grown at 17 °C for 13 h then grown at 28 °C in YEM media

Reached half of O.D. 600 nm of Standard

Heat shock

Grown at 40 °C for 11 h then grown at 28 °C in YEM media

Same as above

Low pH

Grown at 28 °C in AB5.5 media [50]

Reached mid-log phase

Low Iron

Grown at 28 °C in AB7 without Fe media (AB7 Fe-) [51]

Same as above

Oxidative

Grown at 28 °C in 40 mL YEM media with 130 μL 1 % Hydrogen peroxide

Reached half of O.D. 600 nm of Standard

Cold shock & oxidative

Grown at 17 °C for 13 h then grown at 28 °C in YEM media

Reached quarter of O.D. 600 nm of Standard

Low pH & cold shock

Grown at 17 °C for 13 h then grown at 28 °C in AB5.5 media

Same as above

Low pH & heat shock

Grown at 40 °C for 11 h then grown at 28 °C in AB5.5 media

Same as above

Low pH & low Iron

Grown at 28 °C in AB5.5 without Fe media (AB5.5 Fe-)

Same as above