Fig. 1
From: The effect of DNA extraction methodology on gut microbiota research applications
Effect of DNA extraction method on double stranded DNA yield, nucleic acids quality metrics and quantitative PCR analysis. The CHAO method gave the maximum concentration of amplicons per mass (g) of faecal samples (a). While PHEC method had the highest double stranded DNA concentration (bottom left) for the probiotic capsule, the absorbance ratio 260/280 indicated that the extracted nucleic acids were of low purity (top left). Right panel (b) shows the 16S rRNA amplicon copies in qPCR analysis. ED, AB, IM correspond to the three faecal sample and VSL to the proprietary probiotic capsule ID respectively