Table 2 Organisms used for cross-checking of primers to ensure lack of cross-species and genera reactivity
From: Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae
Organism | Source | Specimen type | Number of specimens analyzed |
|---|---|---|---|
Plasmodium falciparum | Reference laboratory | Whole blood | 4 |
P. vivax | Reference laboratory | Whole blood | 5 |
P. ovale | Reference laboratory | Whole blood | 3 |
P. malariae | Reference laboratory | Whole blood | 1 |
Entamoeba histolytica | Reference laboratory | Stool | 1 |
Herpes simplex 1 | Reference laboratory | DNA from viral culture | 1 |
Herpes simplex 2 | Reference laboratory | DNA from viral culture | 1 |
Rickettsiales | Reference laboratory | Acanthamoeba culture, endosymbiont | 1 |
Chlamydiales | Reference laboratory | Acanthamoeba culture, endosymbiont | 1 |
Legionellales | Reference laboratory | Clinical culture isolate | 1 |