Table 1 The oligonucleotides listed were applied in RT-PCRs to validate non-canonical splice sites selected candidate genes in Nd-1
Name | Gene | Sequence | Length | Orientation | Recommended annealing temperature [°C] |
|---|---|---|---|---|---|
S015 | At1g79350 (FGT1) | GCTTCCCTGGAGTGCTGATCG | 21 | Forward | 61 |
S016 | At1g79350 (FGT1) | TCGGGTTCATCAATCGAGCATCC | 23 | Reverse | 61 |
S017 | At1g79350 (FGT1) | AAGAACAGGTAGTTTCTCCTGCTCC | 25 | Reverse | 60 |
S003 | At4g01800 (AGY1) | ACTGGTGAAGGGAAAACGCTTG | 22 | Forward | 59 |
S004 | At4g01800 (AGY1) | AATGTATATCCCGCTCAAAGGCTG | 24 | Reverse | 59 |
S005 | At4g01800 (AGY1) | TCTTCTGCTTTTCATCAACAGTGTAATG | 28 | Reverse | 58 |
S018 | At4g27500 (PPI1) | AGCCGCAGAAGGAAGAAAAGC | 21 | Forward | 59 |
S019 | At4g27500 (PPI1) | ACGCGATGAGACGAATTCCGAG | 22 | Forward | 61 |
S020 | At4g27500 (PPI1) | CTCTTGGGATCGTTTCTGGTCC | 22 | Reverse | 59 |