Table 1 Optimization of different parameters in SD polymerase based and Taq DNA polymerase-based T-ARMS PCR genotyping
From: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
Parameters | SD polymerase based T-ARMS (SNP rs445709131) | Taq DNA polymerase based T-ARMS PCR (SNP rs445709131) |
|---|---|---|
DNA polymerase | 2 Units | 1 Unit |
Outer: inner primer ratio | 2:1 | 1:2 |
Annealing temperature (°C) | 50–60 | 55 |
Genomic DNA quantity (ng) | 50–100 | 80–100 |
Need of PCR enhancers | Not required | DMSO (5%) |
MgCl2 (mM) | 1.5–2 | 2 |
No. of PCR cycles | 25 | 35 |