Fig. 1From: Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligasea Nucleotide sequence and translation of the N-terminal Avi-TEV-3xFLAG tandem affinity tag, cloned as an Acc65I/XhoI fragment in pBluescript SK (see also Additional file 1: Figure S1A). The Kozak sequence is underlined. b Nucleotide sequence and translation of the C-terminal 3xFLAG-TEV-Avi tandem affinity tag which was cloned as an EcoRI/HindIII fragment in pBluescript KS (see also Additional file 1: Figure S1B). Asterisks denote stop codonsBack to article page