Fig. 2
From: CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition
Establishment of the LMβ1::Dendra2 clonal line. a Diagram highlighting the screening workflow of > 500 single cell clones. Cells were expanded from single cells then screened based on green protein expression. Green clones were expanded then further screened using PCR. b Representative PCR gel from secondary screen on green clones using primers designed to give two potential products, a short (274 bp) and a full (967 bp) product in successfully edited cells only. c Positive clone 59B2 was sequenced to confirm for the presence of Dendra2. Start of the Dendra2 insert shown in SnapGene (LMβ1::Dendra2), with the sequence for exon34 and the 3′UTR of LMβ1 shown for reference