Fig. 2

Lowering mamA levels leads to cell filamentation and asymmetric DNA distribution. a DIC Microscopy showing WT (upper panels and mamA KD no ATc) and mamA depleted (mamA KD +ATc) phenotypes. b Cell length distribution determined by microscopy and analyzed with ImageJ software. c DIC microscopy and DNA staining with SYTO 24 in mamA depleted (+ ATc) and non-depleted (no ATc) cells. d Quantification of DNA occupation observed in SYTO 24-stained cells. Percent DNA occupation was calculated by dividing length of DNA fluorescence by cell length. ****p < 0.0001 Kruskal–Wallis followed by Dunn’s multiple comparisons test