Fig. 2

Introduction of the CRISPR/Cas9 system into embryos at different development stages by microinjection and electroporation. a Genotype of blastocysts. The proportions were calculated by dividing the number of gene-edited (bi-allelic and mosaic) blastocysts by the total number of analyzed blastocysts. bi: blastocysts having bi-allelic mutations, mos: blastocysts having mosaic mutations. b Mutation efficiency of gene-edited blastocysts. The mean proportions represent the proportion of indel mutation events in gene-edited blastocysts. The numbers within parentheses under the X-axis indicate the total number of examined samples. Error bars indicate mean ± SEM b. ^{a−b, A−C}Bars with different letters differ significantly (P < 0.05). MI: microinjection, EP: electroporation