Fig. 1

Effects of resveratrol (RSV) on T. gondii replication and H3 acetylation a Effect of RSV on intracellular RH tachyzoite growth. A dose-dependent growth (0-100 μM) curve relative to RFP fluorescence after 24 h of treatment is shown. Three independent experiments were performed in triplicate each time, and the data are presented as mean ± standard deviation (SD). b hTERT cell monolayers were incubated for 24 h in the absence or presence of RSV. Cytotoxicity was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide reduction (with absorbance measured at 540 nm), which is presented relative to the viability of the untreated controls (defined as 100% viability). Determinations were performed in triplicate. The results are representative of three independent experiments. Untreated controls were incubated with 0.5% v/v DMSO. c Indirect immunofluorescence with anti-H3ac antibody (1:200). PV with two parasites were chosen to compare treatment and vehicle (white circle). The upper right white lines correspond to the scale bar: 10 µm. Nuclei were stained with DAPI. Intracellular tachyzoites were treated with RSV (100 μM) or DMSO 0.5% for 24 h. DAPI and fluorescence intensities were quantified from 10 nuclei (Additional file 1) and plotted as relative intensities. The panel and graph are representative of three independent experiments with similar results. d Western blot of T. gondii lysates with anti-H3 (1:250) or anti-H3ac (1:500) antibodies. Lysates were obtained from purified intracellular tachyzoites treated with RSV (50-70 μM) or DMSO 0.5% for 24 h. H3ac band intensities were quantified relative to histone H3 band intensity and plotted. Statistical analysis was performed with one-way ANOVA and Tukey’s multiple comparison test (*p ≤ 0.05 and ****p ≤ 0.0001)