Fig. 2

Effect of resveratrol (RSV) on T. gondii H4K16 acetylation a Indirect immunofluorescence with anti-H4K16ac antibody (1:200). PV with two parasites were chosen to compare treatment and vehicle (white circle). The upper right white lines correspond to the scale bar: 10 µm. Nuclei were stained with DAPI. Intracellular tachyzoites were treated with RSV (100 μM) or DMSO 0.5% for 24 h. DAPI and fluorescence intensities were quantified from 10 nuclei (Additional file 1) and plotted as relative intensities. The panel and graph are representative of three independent experiments with similar results. b Western blot of T. gondii lysates with anti-H4 (1:250) or anti-H4K16ac (1:500) antibodies. Lysates were obtained from purified intracellular tachyzoites treated with RSV (50 μM) or DMSO 0.5% for 24 h. H4K16ac band intensities were quantified relative to histone H4 band intensity and plotted. Statistical analysis was performed with one-way ANOVA and Tukey’s multiple comparison test (*p ≤ 0.05 and ****p ≤ 0.0001)