From: Staphylococcus aureus pigmentation is not controlled by Hfq
Strain name | Key features | Reference or construction |
---|---|---|
RN4220 | Transformable by DNA from E. coli | [25] |
SAPhB5 | RN4220 Δhfq::cat | [13] |
NCTC8325 | Clinical isolate | [26] |
SAPhB224 | NCTC8325 Δhfq::cat | NCTC8325 + ϕ11(SAPhB5) |
NCTC8325-4 | NCTC8325 Δϕ11 Δϕ12 Δϕ13 | [27] |
SAPhB197 | NCTC8325-4 Δhfq::cat | NCTC8325-4 + ϕ11(SAPhB5) |
RN6390 | NCTC 8325-4 ϕ6390 | [28] |
SAPhB22 | RN6390 Δhfq::cat | [13] |
HG001 | NCTC8325 rsbU repaired | [22] |
SAPhB199 | HG001 Δhfq::cat | HG001 + ϕ11(SAPhB5) |
HG002 | NCTC8325 tcaR repaired | [22] |
SAPhB201 | HG002 Δhfq::cat | HG002 + ϕ11(SAPhB5) |
HG003 | NCTC8325 rsbU and tcaR repaired | [22] |
SAPhB203 | HG003 Δhfq::cat | HG003 + ϕ11(SAPhB5) |
COL | Methicillin resistant clinical isolate | [29] |
SAPhB16 | COL Δhfq::cat | [13] |
Newman | Clinical isolate | [30] |
SAPhB17 | Newman Δhfq::cat | [13] |
SAPhB142 | RN4220 pRMC2 | RN4220 + pRMC2 |
SAPhB248 | RN4220 pRMC2Hfq | RN4220 + pRMC2Hfq |
SAPhB251 | RN4220 pRMC2HfqFLAG | RN4220 + pRMC2HfqFLAG |
SAPhB233 | HG003 pRMC2 | HG003 + pRMC2 |
SAPhB249 | HG003 pRMC2Hfq | HG003 + pRMC2Hfq |
SAPhB257 | HG003 pRMC2HfqFLAG | HG003 + pRMC2HfqFLAG |
Plasmid name | Key features | Reference/construction |
---|---|---|
pRMC2 | Anhydrotetracycline (aTc) inducible promoter Pxyl/tetO | [17] |
pRMC2Hfq | hfq inducible expression | See “Methods” |
pRMC2FLAG | pRMC2 derivative for translational gene fusions with 3xflag coding sequence | See “Methods” |
pRMC2HfqFLAG | hfq::3xflag inducible expression | See “Methods” |
Primer name | Sequence | Purpose |
---|---|---|
39 | GGGGTACCATGATTGCAAACGAAAAC | hfq amplification (with a KpnI site) |
49 | GGGGAATTCTTATTCTTCACTTTCAGTAGATGC | hfq amplification (with an EcoRI site) |
856 | GGTACCGTTAACAGATCTGAG | pRMC2 amplification |
918 | GCTTATTTTAATTATACTCTATCAATGATAGAG | pRMC2 and pRMC2FLAG amplifications |
858 | TCAGATCTGTTAACGGTACCGGAATTAGCTTGCATGGAA | 3xflag amplification |
919 | GATAGAGTATAATTAAAATAAGCGAGCTCGACTACAAAGACCA | 3xflag amplification |
865 | GACTACAAAGACCATGACGG | pRMC2FLAG amplification |
939 | GATAGAGTATAATTAAAATAAGCGTAAAAGGAGTCCGACAGATGA | hfq amplification for cloning in pRMC2FLAG |
940 | CCGTCATGGTCTTTGTAGTCTTCTTCACTTTCAGTAGATGCTTG | hfq amplification for cloning in pRMC2FLAG |