Table 1 The PCR components and concentrations
Reagents | Concentration | Volume |
|---|---|---|
H2O | 5.5 µl | |
Buffer fail safe J | 2.5 U/ µl | 10 µl |
Primer F | 10 µM | 1 µl |
Primer R | 10 µM | 1 µl |
Taq FailSafe | 2.5 U/ µl | 0.5 µl |
DNA | 50 ng/ µl | 2 µl |
Reagents | Concentration | Volume |
|---|---|---|
H2O | 5.5 µl | |
Buffer fail safe J | 2.5 U/ µl | 10 µl |
Primer F | 10 µM | 1 µl |
Primer R | 10 µM | 1 µl |
Taq FailSafe | 2.5 U/ µl | 0.5 µl |
DNA | 50 ng/ µl | 2 µl |