From: Spontaneous miscarriage driven by maternal genetic mutation at position of PAI-1-844G/A: shed light on a race-specific genetic polymorphism
Cycles
Step
Temperature (°C)
Time
1 cycle
Initial Denaturation
94
5 min
Denaturation
95
15 S
35 cycle
Annealing:
MTHFR
60
30 S
PAI-1
55
45 S
Factor V Leiden
40 S
Extension
72
Final Extension
10 min
Hold
4