Table 1 Specific PCR primers for the detection of genes used in this study
Gene | Primer | Sequence (5’-3’) | Size (bp)/Annealing temp. | References |
|---|---|---|---|---|
blaOXA−51 | F | CTA ATA ATT GAT CTA CTC AAG TTA C | 988/56.5 | [15] |
R | GAA TAC TCC ATT TGA ACC ART GG | |||
rpoB | F | CTG ACT TGA CGC GTG A | 1024/57.0 | |
R | TGT TTG AAC CCA TGA GC | |||
gluconolactonase | F | TTG GAG AAT GCC CAA CTT GG | 185/56.5 | [20] |
R | CCC GTC TTC GAG CGC AAC | |||
intI1 | F | CAG TGG ACA TAA GCC TGT TC | 160/58.0 | [15] |
R | CCC GAG GCA TAG ACT GTA | |||
CS | F | GGC ATC CAA GCA GCA AG | Variable/58.0 | [15] |
R | AAG CAG ACT TGA CCT GA | |||
blaVIM | F | GAT GGT GTT TGG TCG CAT A | 390/55.0 | |
R | CGA ATG CGC AGC ACC AG | |||
blaIMP | F | GGA ATA GAG TGG CTT AAY TCT C | 232/55.0 | |
R | GGT TTA AYA AAA CAA CCA CC | |||
blaNDM | F | GGT TTG GCG ATC TGG TTT TC | 621/52.0 | [22] |
R | CGG AAT GGC TCA TCA CGA TC |