Figure 4

Studies with serum free suspension adapted BHK21 cells comparing the different UCOE plasmids. Suspension adapted BHK21 cells were transfected with equimolar amounts of the UCOE plasmids listed in figure 1 and the FVIII activity/ml (A), and the BDD protein levels secreted as measured by ELISA (B) were measured. After these cells were cultured under hygromycin selection for one month, the levels of BDD mRNA (C), secreted protein (D) and secreted activity (E) were measured. Data was statistically analyzed using 't' test to detect significant differences between the 3.2 Kb and the 1.5 Kb UCOE containing expression constructs (*: p < 0.05 or more).