Skip to main content

Editor's choice collection

qPCR: Applications and methodologies

This retrospective article collection has been edited by Dr. Diego A. Forero, MD, PhD. 

The analytical methods based on quantitative polymerase chain reaction (qPCR) are broadly used around the world for multiple applications, due to their flexibility, sensitivity and cost. An important number of qPCR-based methods have been developed for analysis of genetic variants, gene expression and DNA methylation, in humans, animals, plants and microorganisms.
In this Editor’s choice collection, we highlight several key papers previously published in BMC Research Notes about methodological advances and applications of qPCR-based techniques. Studies range from exploring the effect of master mixes, to inhibitors of qPCR, DNA extraction methods, novel methods for assessing DNA methylation, and the analysis of siRNA-mediated mRNA silencing.

This collection was published 30 July 2021. 

The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
Jianxin Yang, Berit Kemps-Mols, Marijke Spruyt-Gerritse, Jacqueline Anholts, Frans Claas & Michael Eikmans 
BMC Research Notes 9, 292 (2016) 

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon
Jim F Huggett, Tanya Novak, Jeremy A Garson, Clare Green, Stephen D Morris-Jones, Robert F Miller & Alimuddin Zumla 
BMC Research Notes 1, 70 (2008)

Leukocyte telomere length variation due to DNA extraction method
Joshua Denham, Francine Z Marques & Fadi J Charchar 
BMC Research Notes 7, 877 (2014) 

A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation
M Yat Tse, Janet E Ashbury, Nora Zwingerman, Will D King, Sherry AM Taylor & Stephen C Pang 
BMC Research Notes 4, 565 (2011)

A new approach to primer design for the control of PCR bias in methylation studies
Tomasz K Wojdacz, Lise Lotte Hansen & Alexander Dobrovic 
BMC Research Notes 1, 54 (2008)

The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
Mike Herbert, Natacha Coppieters, Annette Lasham, Helen Cao & Glen Reid 
BMC Research Notes 4, 148 (2011) 

Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design
Katherine Holmes, Catrin M Williams, Elinor A Chapman & Michael J Cross 
BMC Research Notes 3, 53 (2010)

Analysis options for high-throughput sequencing in miRNA expression profiling
Tomasz Stokowy, Markus Eszlinger, Michał Świerniak, Krzysztof Fujarewicz, Barbara Jarząb, Ralf Paschke & Knut Krohn 
BMC Research Notes 7, 144 (2014) 

RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts
Joëlle Vermeulen, Stefaan Derveaux, Steve Lefever, Els De Smet, Katleen De Preter, Nurten Yigit, Anne De Paepe, Filip Pattyn, Frank Speleman & Jo Vandesompele 
BMC Research Notes 2, 235 (2009)

New Content ItemDr. Diego A. Forero, MD, PhD is currently Professor and Research Leader at the School of Health and Sport Sciences, Fundación Universitaria del Área Andina (Bogotá, Colombia). He is a Medical Doctor (Universidad Nacional de Colombia, Colombia) and has a PhD in Biomedical Sciences (University of Antwerp, Belgium). Dr. Forero has produced a significant amount of research in human genetics and genomics, with over 100 international scientific publications. He is a Senior Editorial Board Member for BMC Research Notes and has been a peer reviewer for numerous international publications, as well as academic and scientific institutions.

Annual Journal Metrics